IFOM
  1. Home
  2. Research
  3. Technological Platforms
  4. Advanced Light Microscopy
  5. Sara Barozzi

Advanced Light Microscopy

Microscopia Ottica Avanzata

Sara Barozzi

Coordinator laboratory for advanced light microscopy facility

Sara Barozzi obtained her Master’s degree in Biological Sciences with a focus on molecular biology in 2000. She began her research career in Pier Giuseppe Pelicci’s lab, studying the interaction between p66SHC and p53 and their role in mitochondrial activity.
With a long-standing interest in microscopy, she joined the Imaging Facility at IEO in 2004 and then moved to IFOM’s Advanced Light Microscopy Unit in 2014.
Sara has extensive experience in sample preparation and live-cell imaging, providing comprehensive support to researchers throughout all stages of imaging experiments—from planning to acquisition. She is skilled in a range of advanced microscopy techniques used in cancer research and is also responsible for the microinjection service.

Publications

Publications Pre-IFOM
<
Publications Pre-IFOM
  1. Trinei M, Giorgio M, Barozzi S, Pelicci PG. The Oxidative Stress Response Gene P66 and the Tumor Suppressor Gene P53 Induce Mitochondrial Dna Damages. ScientificWorldJournal. 2001 Oct 12;1:127. doi: 10.1100/tsw.2001.230. PMID: 30147606; PMCID: PMC6084532.
  2. Trinei M, Giorgio M, Cicalese A, Barozzi S, Ventura A, Migliaccio E, Milia E, Padura IM, Raker VA, Maccarana M, Petronilli V, Minucci S, Bernardi P, Lanfrancone L, Pelicci PG. A p53-p66Shc signalling pathway controls intracellular redox status, levels of oxidation-damaged DNA and oxidative stress-induced apoptosis. Oncogene. 2002 May 30;21(24):3872-8. doi: 10.1038/sj.onc.1205513. PMID: 12032825.
  3. Schneider M, Barozzi S, Testa I, Faretta M, Diaspro A. Two-photon activation and excitation properties of PA-GFP in the 720-920-nm region. Biophys J. 2005 Aug;89(2):1346-52. doi: 10.1529/biophysj.104.054502. Epub 2005 May 20. PMID: 15908572; PMCID: PMC1366619.
  4. Diaspro A, Testa I, Faretta M, Magrassi R, Barozzi S, Parazzoli D, Vicidomini G. 3D localized photoactivation of pa-GFP in living cells using two-photon interactions. Conf Proc IEEE Eng Med Biol Soc. 2006;2006:389-91. doi: 10.1109/IEMBS.2006.259967. PMID: 17946398.
  5. Testa I, Garrè M, Parazzoli D, Barozzi S, Ponzanelli I, Mazza D, Faretta M, Diaspro A. Photoactivation of pa-GFP in 3D: optical tools for spatial confinement. Eur Biophys J. 2008 Sep;37(7):1219-27. doi: 10.1007/s00249-008-0317-9. Epub 2008 Apr 1. PMID: 18379772.
  6. Testa I, Garrè M, Parazzoli D, Barozzi S, Ponzanelli I, Mazza D, Faretta M, Diaspro A. Photoactivation of pa-GFP in 3D: optical tools for spatial confinement. Eur Biophys J. 2008 Sep;37(7):1219-27. doi: 10.1007/s00249-008-0317-9. Epub 2008 Apr 1. PMID: 18379772.
  7. Palamidessi A, Testa I, Frittoli E, Barozzi S, Garrè M, Mazza D, Di Fiore PP, Diaspro A, Scita G, Faretta M. Understanding biological dynamics: following cells and molecules to track functions and mechanisms. Eur Biophys J. 2010 May;39(6):947-57. doi: 10.1007/s00249-009-0461-x. Epub 2009 May 20. PMID: 19455321.
  8. Saccheri F, Pozzi C, Avogadri F, Barozzi S, Faretta M, Fusi P, Rescigno M. Bacteria-induced gap junctions in tumors favor antigen cross-presentation and antitumor immunity. Sci Transl Med. 2010 Aug 11;2(44):44ra57. doi: 10.1126/scitranslmed.3000739. PMID: 20702856.
  9. Fumagalli M, Rossiello F, Clerici M, Barozzi S, Cittaro D, Kaplunov JM, Bucci G, Dobreva M, Matti V, Beausejour CM, Herbig U, Longhese MP, d'Adda di Fagagna F. Telomeric DNA damage is irreparable and causes persistent DNA-damage-response activation. Nat Cell Biol. 2012 Mar 18;14(4):355-65. doi: 10.1038/ncb2466. Erratum in: Nat Cell Biol. 2012 May;14(5):555. PMID: 22426077; PMCID: PMC3717580.
Publications IFOM
  1. Monticelli M, Conca DV, Albisetti E, Torti A, Sharma PP, Kidiyoor G, Barozzi S, Parazzoli D, Ciarletta P, Lupi M, Petti D, Bertacco R. Magnetic domain wall tweezers: a new tool for mechanobiology studies on individual target cells. Lab Chip. 2016 Aug 7;16(15):2882-90. doi: 10.1039/c6lc00368k. Epub 2016 Jul 1. PMID: 27364187.
  2. Peretti M, Raciti FM, Carlini V, Verduci I, Sertic S, Barozzi S, Garré M, Pattarozzi A, Daga A, Barbieri F, Costa A, Florio T, Mazzanti M. Mutual Influence of ROS, pH, and CLIC1 Membrane Protein in the Regulation of G1-S Phase Progression in Human Glioblastoma Stem Cells. Mol Cancer Ther. 2018 Nov;17(11):2451-2461. doi: 10.1158/1535-7163.MCT-17-1223. Epub 2018 Aug 22. PMID: 30135216.
  3. Kidiyoor GR, Li Q, Bastianello G, Bruhn C, Giovannetti I, Mohamood A, Beznoussenko GV, Mironov A, Raab M, Piel M, Restuccia U, Matafora V, Bachi A, Barozzi S, Parazzoli D, Frittoli E, Palamidessi A, Panciera T, Piccolo S, Scita G, Maiuri P, Havas KM, Zhou ZW, Kumar A, Bartek J, Wang ZQ, Foiani M. ATR is essential for preservation of cell mechanics and nuclear integrity during interstitial migration. Nat Commun. 2020 Sep 24;11(1):4828. doi: 10.1038/s41467-020-18580-9. PMID: 32973141; PMCID: PMC7518249.
  4. Giovarelli M, Zecchini S, Martini E, Garrè M, Barozzi S, Ripolone M, Napoli L, Coazzoli M, Vantaggiato C, Roux-Biejat P, Cervia D, Moscheni C, Perrotta C, Parazzoli D, Clementi E, De Palma C. Drp1 overexpression induces desmin disassembling and drives kinesin-1 activation promoting mitochondrial trafficking in skeletal muscle. Cell Death Differ. 2020 Aug;27(8):2383-2401. doi: 10.1038/s41418-020-0510-7. Epub 2020 Feb 10. PMID: 32042098; PMCID: PMC7370230.
  5. Pessina F, Giavazzi F, Yin Y, Gioia U, Vitelli V, Galbiati A, Barozzi S, Garre M, Oldani A, Flaus A, Cerbino R, Parazzoli D, Rothenberg E, d'Adda di Fagagna F. Functional transcription promoters at DNA double-strand breaks mediate RNA-driven phase separation of damage-response factors. Nat Cell Biol. 2019 Oct;21(10):1286-1299. doi: 10.1038/s41556-019-0392-4. Epub 2019 Sep 30. PMID: 31570834; PMCID: PMC6859070.
  6. Colombo A, Fontani M, Dragonetti C, Roberto D, Williams JAG, Scotto di Perrotolo R, Casagrande F, Barozzi S, Polo S. A Highly Luminescent Tetrahydrocurcumin IrIII Complex with Remarkable Photoactivated Anticancer Activity. Chemistry. 2019 Jun 12;25(33):7948-7952. doi: 10.1002/chem.201901527. Epub 2019 May 13. PMID: 30985041.
  7. Gritti I, Basso V, Rinchai D, Corigliano F, Pivetti S, Gaviraghi M, Rosano D, Mazza D, Barozzi S, Roncador M, Parmigiani G, Legube G, Parazzoli D, Cittaro D, Bedognetti D, Mondino A, Segalla S, Tonon G. Loss of ribonuclease DIS3 hampers genome integrity in myeloma by disrupting DNA:RNA hybrid metabolism. EMBO J. 2022 Nov 17;41(22):e108040. doi: 10.15252/embj.2021108040. Epub 2022 Oct 10. PMID: 36215697; PMCID: PMC9670201.
  8. Hershey BJ, Barozzi S, Orsenigo F, Pompei S, Iannelli F, Kamrad S, Matafora V, Pisati F, Calabrese L, Fragale G, Salvadori G, Martini E, Totaro MG, Magni S, Guan R, Parazzoli D, Maiuri P, Bachi A, Patil KR, Cosentino Lagomarsino M, Havas KM. Clonal cooperation through soluble metabolite exchange facilitates metastatic outgrowth by modulating Allee effect. Sci Adv. 2023 Sep 15;9(37):eadh4184. doi: 10.1126/sciadv.adh4184. Epub 2023 Sep 15. PMID: 37713487; PMCID: PMC10881076.
  9. Bastianello G, Porcella G, Beznoussenko GV, Kidiyoor G, Ascione F, Li Q, Cattaneo A, Matafora V, Disanza A, Quarto M, Mironov AA, Oldani A, Barozzi S, Bachi A, Costanzo V, Scita G, Foiani M. Cell stretching activates an ATM mechano-transduction pathway that remodels cytoskeleton and chromatin. Cell Rep. 2023 Dec 26;42(12):113555. doi: 10.1016/j.celrep.2023.113555. Epub 2023 Dec 11. PMID: 38088930.
  10. Popęda M, Kowalski K, Wenta T, Beznoussenko GV, Rychłowski M, Mironov A, Lavagnino Z, Barozzi S, Richert J, Bertolio R, Myszczyński K, Szade J, Bieńkowski M, Miszewski K, Matuszewski M, Żaczek AJ, Braga L, Del Sal G, Bednarz-Knoll N, Maiuri P, Nastały P. Emerin mislocalization during chromatin bridge resolution can drive prostate cancer cell invasiveness in a collagen-rich microenvironment. Exp Mol Med. 2024 Sep;56(9):2016-2032. doi: 10.1038/s12276-024-01308-w. Epub 2024 Sep 2. PMID: 39218980; PMCID: PMC11446916.
  11. Gioia U, Tavella S, Martínez-Orellana P, Cicio G, Colliva A, Ceccon M, Cabrini M, Henriques AC, Fumagalli V, Paldino A, Presot E, Rajasekharan S, Iacomino N, Pisati F, Matti V, Sepe S, Conte MI, Barozzi S, Lavagnino Z, Carletti T, Volpe MC, Cavalcante P, Iannacone M, Rampazzo C, Bussani R, Tripodo C, Zacchigna S, Marcello A, d'Adda di Fagagna F. SARS-CoV-2 infection induces DNA damage, through CHK1 degradation and impaired 53BP1 recruitment, and cellular senescence. Nat Cell Biol. 2023 Apr;25(4):550-564. doi: 10.1038/s41556-023-01096-x. Epub 2023 Mar 9. PMID: 36894671; PMCID: PMC10104783.
  12. Nespolo A, Stefenatti L, Pellarin I, Gambelli A, Rampioni Vinciguerra GL, Karimbayli J, Barozzi S, Orsenigo F, Spizzo R, Nicoloso MS, Segatto I, D'Andrea S, Bartoletti M, Lucia E, Giorda G, Canzonieri V, Puglisi F, Belletti B, Schiappacassi M, Baldassarre G, Sonego M. USP1 deubiquitinates PARP1 to regulate its trapping and PARylation activity. Sci Adv. 2024 Nov 15;10(46):eadp6567. doi: 10.1126/sciadv.adp6567. Epub 2024 Nov 13. PMID: 39536107; PMCID: PMC11559621.
  13. Curti L, Rohban S, Bianchi N, Croci O, Andronache A, Barozzi S, Mattioli M, Ricci F, Pastori E, Sberna S, Bellotti S, Accialini A, Ballarino R, Crosetto N, Wade M, Parazzoli D, Campaner S. CDK12 controls transcription at damaged genes and prevents MYC-induced transcription-replication conflicts. Nat Commun. 2024 Aug 18;15(1):7100. doi: 10.1038/s41467-024-51229-5. PMID: 39155303; PMCID: PMC11330984.
Sara Barozzi

Facility members

Dario Parazzoli, Head of facility

Zeno Lavagnino

Serena Magni

Fabrizio Orsenigo

Mattia Pavani

Reference PI:

Giorgio Scita