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Functional Genomics Screening

Screening Genomico Funzionale

Mario Cinquanta

Functional genomics screening coordinator

Mario Cinquanta graduated with honors in Pharmaceutical Chemistry and Technology from the University of Milan and obtained a postgraduate specialization in Pharmacological Research at the Mario Negri Institute. He has extensive experience in biomedical research, ranging from neuropharmacology to molecular genetics and experimental oncology.

After training and research appointments in Italy and abroad, including a research position at the National Institutes of Health (NIH, Bethesda, USA) in the laboratory of Nobel laureate Marshall Nirenberg, he continued his career at the European Institute of Oncology and later at IFOM in Milan.

In recent years, he has managed advanced technological platforms, first in the field of monoclonal antibodies and subsequently in genome editing, contributing to collaborative research projects and the implementation of innovative functional genomic screening approaches.

Publications

Publications Pre-IFOM
  1. Cattabeni, F., M. Cinquanta and M. Di Luca (1994). "Protein kinase C-dependent phosphorylation in prenatally induced microencephaly." Neurotoxicology 15(1): 161-169.
  2. Di Luca, M., A. Caputi, M. Cinquanta, M. Cimino, P. Marini, A. Princivalle, P. N. De Graan, W. H. Gispen and F. Cattabeni (1995). "Changes in protein kinase C and its presynaptic substrate B-50/GAP-43 after intrauterine exposure to methylazoxy-methanol, a treatment inducing cortical and hippocampal damage and cognitive deficit in rats." Eur J Neurosci 7(5): 899-906.
  3. Mennini, T., M. Gobbi, D. Crespi, M. Cinquanta, E. Frittoli, P. Giorcelli, M. Anelli and S. Caccia (1996). "In vivo and in vitro interaction of flunarizine with D-fenfluramine serotonergic effects." Pharmacol Biochem Behav 53(1): 155-161.
  4. Cinquanta, M., E. Frittoli, T. Mennini and M. Gobbi (1997). "Further evidence of Ca(2+)-dependent, exocytotic-like serotonin release induced by D-fenfluramine." Pharmacol Res 35(5): 439-442.
  5. Cinquanta, M., T. Ratovitski, D. Crespi, M. Gobbi, T. Mennini and R. Simantov (1997). "Carrier-mediated serotonin release induced by d-fenfluramine: studies with human neuroblastoma cells transfected with a rat serotonin transporter." Neuropharmacology 36(6): 803-809.
  6. Foddi, M. C., M. Cinquanta and T. Mennini (1997). "Effect of psychological stress on [35S]TBPS binding in rat brain." Pharmacol Biochem Behav 58(2): 373-377.
  7. Rovescalli, A. C., M. Cinquanta, J. Ferrante, C. A. Kozak and M. Nirenberg (2000). "The mouse Nkx-1.2 homeobox gene: alternative RNA splicing at canonical and noncanonical splice sites." Proc Natl Acad Sci U S A 97(5): 1982-1987.
  8. Cinquanta, M., A. C. Rovescalli, C. A. Kozak and M. Nirenberg (2000). "Mouse Sebox homeobox gene expression in skin, brain, oocytes, and two-cell embryos." Proc Natl Acad Sci U S A 97(16): 8904-8909.
  9. Ornstein, D. K., M. Cinquanta, S. Weiler, P. H. Duray, M. R. Emmert-Buck, C. D. Vocke, W. M. Linehan and J. A. Ferretti (2001). "Expression studies and mutational analysis of the androgen regulated homeobox gene NKX3.1 in benign and malignant prostate epithelium." J Urol 165(4): 1329-1334.
Publications IFOM
  1. Deflorian, G., M. Cinquanta, C. Beretta, A. Venuto, C. Santoriello, D. Baldessari, F. Pezzimenti, M. Aliprandi, M. Mione and A. de Marco (2009). "Monoclonal antibodies isolated by large-scale screening are suitable for labeling adult zebrafish (Danio rerio) tissues and cell structures." J Immunol Methods 346(1-2): 9-17.
  2. Gruszka, A. M., S. Lavorgna, M. I. Consalvo, T. Ottone, C. Martinelli, M. Cinquanta, G. Ossolengo, G. Pruneri, F. Buccisano, M. Divona, M. Cedrone, E. Ammatuna, A. Venditti, A. de Marco, F. Lo-Coco and P. G. Pelicci (2010). "A monoclonal antibody against mutated nucleophosmin 1 for the molecular diagnosis of acute myeloid leukemias." Blood 116(12): 2096-2102.
  3. Buckle, A. M., M. A. Bate, S. Androulakis, M. Cinquanta, J. Basquin, F. Bonneau, D. K. Chatterjee, D. Cittaro, S. Graslund, A. Gruszka, R. Page, S. Suppmann, J. X. Wheeler, D. Agostini, M. Taussig, C. F. Taylor, S. P. Bottomley, A. Villaverde and A. de Marco (2011). "Recombinant protein quality evaluation: proposal for a minimal information standard." Stand Genomic Sci 5(2): 195-197.
  4. Segre, C. V., S. Senese, S. Loponte, S. Santaguida, P. Soffientini, G. Grigorean, M. Cinquanta, G. Ossolengo, C. Seiser and S. Chiocca (2016). "A monoclonal antibody specific for prophase phosphorylation of histone deacetylase 1: a readout for early mitotic cells." MAbs 8(1): 37-42.
  5. Gerboth S, Frittoli E, Palamidessi A, Baltanas FC, Salek M, Rappsilber J, Giuliani C, Troglio F, Rolland Y, Pruneri G, Kreutmair S, Pallavicini I, Zobel M, Cinquanta M, Minucci S, Gomez C, Santos E, Illert AL, Scita G. Phosphorylation of SOS1 on tyrosine 1196 promotes its RAC GEF activity and contributes to BCR-ABL leukemogenesis. Leukemia. 2018 Mar;32(3):820-827. doi: 10.1038/leu.2017.267. Epub 2017 Aug 18. PMID: 28819285; PMCID: PMC5739283.
  6. Varone, E., Pozzer, D., Di Modica, S., Chernorudskiy, A., Nogara, L., Baraldo, M., Cinquanta, M., Fumagalli, S., Villar-Quiles, R.N., De Simoni, M.G., et al. (2019). SELENON (SEPN1) protects skeletal muscle from saturated fatty acid-induced ER stress and insulin resistance. Redox Biol 24, 101176.
  7. Grelloni C, Garraffo R, Setti A, Rossi F, Peruzzi G, Cinquanta M, Di Rosa MC, Pierotti MA, Beltran M, Bozzoni I. BRCA1 levels and DNA-damage response are controlled by the competitive bindingof circHIPK3 or FMRP to the BRCA1 mRNA. Mol Cell. 2024 Nov 7;84(21):4079-4094.e10. doi: 10.1016/j.molcel.2024.09.016. Epub 2024 Oct 9. PMID: 39389065.
  8. Hariprakash JM, Salviato E, La Mastra F, Sebestyén E, Tagliaferri I, Silva RS, Lucini F, Farina L, Cinquanta M, Rancati I, Riboni M, Minardi SP, Roz L, Gorini F, Lanzuolo C, Casola S, Ferrari F. Leveraging Tissue-Specific Enhancer-Target Gene Regulatory Networks Identifies Enhancer Somatic Mutations That Functionally Impact Lung Cancer. Cancer Res. 2024 Jan 2;84(1):133-153. doi: 10.1158/0008-5472.CAN-23-1129. PMID: 37855660; PMCID: PMC10758689.
  9. Pennacchio FA, Poli A, Pramotton FM, Lavore S, Rancati I, Cinquanta M, Vorselen D, Prina E, Romano OM, Ferrari A, Piel M, Cosentino Lagomarsino M, Maiuri P. N2FXm, a method for joint nuclear and cytoplasmic volume measurements, unravels the osmo-mechanical regulation of nuclear volume in mammalian cells. Nat Commun. 2024 Feb 7;15(1):1070. doi: 10.1038/s41467-024-45168-4. PMID: 38326317; PMCID: PMC10850064.
  10. Grelloni C, Garraffo R, Setti A, Rossi F, Peruzzi G,Cinquanta M, Di Rosa MC, Pierotti MA, Beltran M, Bozzoni I. BRCA1 levels and DNA-damage response are controlled by the competitive binding of circHIPK3 or FMRP to the BRCA1 mRNA. Mol Cell. 2024 Nov 7;84(21):4079-4094.e10. doi: 10.1016/j.molcel.2024.09.016. Epub 2024 Oct 9.PMID:39389065
Mario Cinquanta

Facility members

Giuseppina D'Alessandro, Responsabile della Core Facility

Marisa Aliprandi

Reference PI

Ylli Doksani