IFOM Publications Simona Polo
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Publications Simona Polo

  1. Gahlot P, Kravic B, Rota G, van den Boom J, Levantovsky S, Schulze N, Maspero E, Polo S, Behrends C, Meyer H.
    Lysosomal damage sensing and lysophagy initiation by SPG20-ITCH.
    Mol Cell. 2024 Apr 18;84(8):1556-1569.e10. doi: 10.1016/j.molcel.2024.02.029. Epub 2024 Mar 18.PMID: 38503285

  2. Barroso-Gomila O, Merino-Cacho L, Muratore V, Perez C, Taibi V, Maspero E, Azkargorta M, Iloro I, Trulsson F, Vertegaal ACO, Mayor U, Elortza F, Polo S, Barrio R, Sutherland JD.
    BioE3 identifies specific substrates of ubiquitin E3 ligases.
    Nat Commun. 2023 Nov 23;14(1):7656. doi: 10.1038/s41467-023-43326-8.PMID: 37996419

  3. Menin L, Weber J, Villa S, Martini E, Maspero E, Niño CA, Cancila V, Poli A, Maiuri P, Palamidessi A, Frittoli E, Bianchi F, Tripodo C, Walters KJ, Giavazzi F, Scita G, Polo S.
    A planar polarized MYO6-DOCK7-RAC1 axis promotes tissue fluidification in mammary epithelia.
    Cell Rep. 2023 Aug 29;42(8):113001. doi: 10.1016/j.celrep.2023.113001. Epub 2023 Aug 16.PMID: 37590133

  4. Taibi V, Polo S, Maspero E.
    Monitoring HECT Ubiquitination Activity In Vitro.
    Methods Mol Biol. 2023;2602:81-92. doi: 10.1007/978-1-0716-2859-1_6.PMID: 36446968

  5. Sicari D, Weber J, Maspero E, Polo S.
    The NEDD4 ubiquitin E3 ligase: a snapshot view of its functional activity and regulation.
    Biochem Soc Trans. 2022 Feb 28;50(1):473-485. doi: 10.1042/BST20210731.PMID: 35129615 Review.

  6. Niño CA, Scotto di Perrotolo R, Polo S.
    Recurrent Spliceosome Mutations in Cancer: Mechanisms and Consequences of Aberrant Splice Site Selection.
    Cancers (Basel). 2022 Jan 7;14(2):281. doi: 10.3390/cancers14020281.PMID: 35053445

  7. Magistrati E, Maestrini G, Niño CA, Lince-Faria M, Beznoussenko G, Mironov A, Maspero E, Bettencourt-Dias M, Polo S.
    Myosin VI regulates ciliogenesis by promoting the turnover of the centrosomal/satellite protein OFD1.
    EMBO Rep. 2022 Feb 3;23(3):e54160. doi: 10.15252/embr.202154160. Epub 2021 Dec 27.PMID: 34957672 Free PMC article.

  8. Fajner V, Giavazzi F, Sala S, Oldani A, Martini E, Napoletano F, Parazzoli D, Cesare G, Cerbino R, Maspero E, Vaccari T, Polo S.
    Hecw controls oogenesis and neuronal homeostasis by promoting the liquid state of ribonucleoprotein particles.
    Nat Commun. 2021 Sep 16;12(1):5488. doi: 10.1038/s41467-021-25809-8. PMID: 34531401

  9. Napoletano F, Ferrari Bravo G, Voto IAP, Santin A, Celora L, Campaner E, Dezi C, Bertossi A, Valentino E, Santorsola M, Rustighi A, Fajner V, Maspero E, Ansaloni F, Cancila V, Valenti CF, Santo M, Artimagnella OB, Finaurini S, Gioia U, Polo S, Sanges R, Tripodo C, Mallamaci A, Gustincich S, d'Adda di Fagagna F, Mantovani F, Specchia V, Del Sal G.
    The prolyl-isomerase PIN1 is essential for nuclear Lamin-B structure and function and protects heterochromatin under mechanical stress.
    Cell Rep. 2021 Sep 14;36(11):109694. doi: 10.1016/j.celrep.2021.109694.

  10. Magistrati E, Polo S.
    Myomics: myosin VI structural and functional plasticity.
    Curr Opin Struct Biol. 2021 Apr;67:33-40. doi: 10.1016/j.sbi.2020.09.005. PMID: 33053464

  11. Niño CA, Wollscheid N, Giangreco G, Maspero E, Polo S.
    USP25 Regulates EGFR Fate by Modulating EGF-Induced Ubiquitylation Dynamics.
    Biomolecules. 2020 Nov 13;10(11):1548. doi: 10.3390/biom10111548. PMID: 33202887

  12. Biancospino M, Buel GR, Niño CA, Maspero E, Scotto di Perrotolo R, Raimondi A, Redlingshöfer L, Weber J, Brodsky FM, Walters KJ, Polo S.
    Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension.
    Nat Commun. 2019 Oct 31;10(1):4974. doi: 10.1038/s41467-019-12855-6. PMID: 31672988

  13. Magistrati E, Polo S.
    Myomics: myosin VI structural and functional plasticity.
    Curr Opin Struct Biol. 2020 Oct 11;67:33-40. doi:10.1016/j.sbi.2020.09.005. Epub ahead of print. PMID: 33053464.

  14. Dhanda AS, Yu C, Lulic KT, Vogl AW, Rausch V, Yang D, Nichols BJ, Kim SH,Polo S, Hansen CG, Guttman JA.
    Listeria monocytogenes Exploits Host Caveolin for Cell-to-Cell Spreading.
    mBio. 2020 Jan 21;11(1):e02857-19. doi:10.1128/mBio.02857-19. PMID: 31964732; PMCID: PMC6974566.

  15. Biancospino M, Buel GR, Niño CA, Maspero E, Scotto di Perrotolo R, Raimondi A, Redlingshöfer L, Weber J, Brodsky FM, Walters KJ, Polo S.
    Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension.
    Nat Commun. 2019 Oct 31;10(1):4974. doi: 10.1038/s41467-019-12855-6. PMID: 31672988; PMCID: PMC6823378.

  16. Wagner W, Lippmann K, Heisler FF, Gromova KV, Lombino FL, Roesler MK, Pechmann Y, Hornig S, Schweizer M, Polo S, Schwarz JR, Eilers J, Kneussel M.
    Myosin VI Drives Clathrin-Mediated AMPA Receptor Endocytosis to Facilitate Cerebellar Long-Term Depression.
    Cell Rep. 2019 Jul 2;28(1):11-20.e9. doi: 10.1016/j.celrep.2019.06.005. PMID: 31269433.

  17. Nepravishta R, Ferrentino F, Mandaliti W, Mattioni A, Weber J, Polo S, Castagnoli L, Cesareni G, Paci M, Santonico E.
    CoCUN, a Novel Ubiquitin Binding Domain Identified in N4BP1.
    Biomolecules. 2019 Jul 17;9(7):284. doi: 10.3390/biom9070284. Erratum in: Biomolecules. 2019 Nov 28;9(12): PMID: 31319543; PMCID: PMC6681339.

  18. Weber J, Polo S and Maspero E.
    HECT E3 Ligases: A Tale With Multiple Facets.
    Front Physiol. 2019 Apr 3;10:370. doi: 10.3389/fphys.2019.00370. eCollection 2019.

  19. Pascolutti R, Algisi V, Conte A, Raimondi A, Pasham M, Upadhyayula S, Gaudin R, Maritzen T, Barbieri E, Caldieri G, Tordonato C, Confalonieri S, Freddi S, Malabarba MG, Maspero E, Polo S, Tacchetti C, Haucke V, Kirchhausen T, Di Fiore PP and Sigismund S.
    Molecularly Distinct Clathrin-Coated Pits Differentially Impact EGFR Fate and Signaling.
    Cell Rep. 2019;27:3049-3061 e6.

  20. Maspero E, Fajner V, Weber J and Polo S.
    Detection of ubiquitinated targets in mammalian and Drosophila models.
    Methods Enzymol. 2019;619:293-318.

  21. Dissmeyer N, Coux O, Rodriguez MS, Barrio R; Core Group Members of PROTEOSTASIS
    PROTEOSTASIS: A European Network to Break Barriers and Integrate Science on Protein Homeostasis.
    Trends Biochem Sci. 2019.

  22. Colombo A, Fontani M, Dragonetti C, Roberto D, Williams JAG, Scotto di Perrotolo R, Casagrande F, Barozzi S and Polo S.
    A Highly Luminescent Tetrahydrocurcumin Ir(III) Complex with Remarkable Photoactivated Anticancer Activity
    Chemistry. 2019;25:7948-7952.

  23. Castagnoli L, Mandaliti W, Nepravishta R, Valentini E, Mattioni A, Procopio R, Iannuccelli M, Polo S, Paci M, Cesareni G and Santonico E.
    Selectivity of the CUBAN domain in the recognition of ubiquitin and NEDD8.
    FEBS J. 2019;286:653-677.

  24. Nino CA, Sala S and Polo S.
    When ubiquitin meets E-cadherin: Plasticity of the epithelial cellular barrier.
    Semin Cell Dev Biol. 2018.

  25. Kam CY, Dubash AD, Magistrati E, Polo S, Satchell KJF, Sheikh F, Lampe PD and Green KJ.
    Desmoplakin maintains gap junctions by inhibiting Ras/MAPK and lysosomal degradation of connexin-43.
    J Cell Biol. 2018;217:3219-3235.

  26. Jackl M, Stollmaier C, Strohaker T, Hyz K, Maspero E, Polo S and Wiesner S.
    β-Sheet Augmentation Is a Conserved Mechanism of Priming HECT E3 Ligases for Ubiquitin Ligation.
    J Mol Biol. 2018;430:3218-3233.

  27. Myant KB, Cammareri P, Hodder MC, Wills J, Von Kriegsheim A, Gyorffy B, Rashid M, Polo S, Maspero E, Vaughan L, Gurung B, Barry E, Malliri A, Camargo F, Adams DJ, Iavarone A, Lasorella A and Sansom OJ.
    HUWE1 is a critical colonic tumour suppressor gene that prevents MYC signalling, DNA damage accumulation and tumour initiation.
    EMBO Mol Med. 2017;9:181-197.

  28. Fajner V, Maspero E and Polo S.
    Targeting HECT-type E3 ligases - insights from catalysis, regulation and inhibitors.
    FEBS Lett. 2017;591:2636-2647.

  29. Caldieri G, Barbieri E, Nappo G, Raimondi A, Bonora M, Conte A, Verhoef L, Confalonieri S, Malabarba MG, Bianchi F, Cuomo A, Bonaldi T, Martini E, Mazza D, Pinton P, Tacchetti C, Polo S, Di Fiore PP and Sigismund S.
    Reticulon 3-dependent ER-PM contact sites control EGFR nonclathrin endocytosis.
    Science. 2017;356:617-624.

  30. Wollscheid HP, Biancospino M, He F, Magistrati E, Molteni E, Lupia M, Soffientini P, Rottner K, Cavallaro U, Pozzoli U, Mapelli M, Walters KJ and Polo S.
    Diverse functions of myosin VI elucidated by an isoform-specific alpha-helix domain.
    Nat Struct Mol Biol. 2016;23:300-308.

  31. Sigismund S and Polo S.
    Strategies to Detect Endogenous Ubiquitination of a Target Mammalian Protein.
    Methods Mol Biol. 2016;1449:143-51.

  32. Savio MG, Wollscheid N, Cavallaro E, Algisi V, Di Fiore PP, Sigismund S, Maspero E and Polo S.
    USP9X Controls EGFR Fate by Deubiquitinating the Endocytic Adaptor Eps15.
    Curr Biol. 2016;26:173-183.

  33. Maspero E and Polo S.
    In Vitro Ubiquitination: Self-Ubiquitination, Chain Formation, and Substrate Ubiquitination Assays.
    Methods Mol Biol. 2016;1449:153-60.

  34. He F, Wollscheid HP, Nowicka U, Biancospino M, Valentini E, Ehlinger A, Acconcia F, Magistrati E, Polo S and Walters KJ.
    Myosin VI Contains a Compact Structural Motif that Binds to Ubiquitin Chains.
    Cell Rep. 2016;14:2683-94.

  35. Capuani F, Conte A, Argenzio E, Marchetti L, Priami C, Polo S, Di Fiore PP, Sigismund S and Ciliberto A.
    Quantitative analysis reveals how EGFR activation and downregulation are coupled in normal but not in cancer cells.
    Nat Commun. 2015;6:7999.

  36. Polo S, Di Fiore PP and Sigismund S.
    Keeping EGFR signaling in check: ubiquitin is the guardian.
    Cell Cycle. 2014;13:681-2.

  37. Persaud A, Alberts P, Mari S, Tong J, Murchie R, Maspero E, Safi F, Moran MF, Polo S and Rotin D.
    Tyrosine phosphorylation of NEDD4 activates its ubiquitin ligase activity.
    Sci Signal. 2014;7:ra95.

  38. Mari S, Ruetalo N, Maspero E, Stoffregen MC, Pasqualato S, Polo S and Wiesner S.
    Structural and functional framework for the autoinhibition of Nedd4-family ubiquitin ligases.
    Structure. 2014;22:1639-49.

  39. Boissan M, Montagnac G, Shen Q, Griparic L, Guitton J, Romao M, Sauvonnet N, Lagache T, Lascu I, Raposo G, Desbourdes C, Schlattner U, Lacombe ML, Polo S, van der Bliek AM, Roux A and Chavrier P.
    Membrane trafficking. Nucleoside diphosphate kinases fuel dynamin superfamily proteins with GTP for membrane remodeling.
    Science. 2014;344:1510-5.

  40. Sorrentino V, Nelson JK, Maspero E, Marques AR, Scheer L, Polo S and Zelcer N.
    IDOL axis defines a clathrin-, caveolae-, and dynamin-independent endocytic route for LDLR internalization and lysosomal degradation.
    J Lipid Res. 2013;54:2174-84.

  41. Sigismund S, Algisi V, Nappo G, Conte A, Pascolutti R, Cuomo A, Bonaldi T, Argenzio E, Verhoef LG, Maspero E, Bianchi F, Capuani F, Ciliberto A, Polo S and Di Fiore PP.
    Threshold-controlled ubiquitination of the EGFR directs receptor fate.
    EMBO J. 2013;32:2140-57.

  42. Nino CA, Chaparro J, Soffientini P, Polo S and Wasserman M.
    Ubiquitination dynamics in the early-branching eukaryote Giardia intestinalis.
    Microbiologyopen. 2013;2:525-39.

  43. Maspero E, Valentini E, Mari S, Cecatiello V, Soffientini P, Pasqualato S and Polo S.
    Structure of a ubiquitin-loaded HECT ligase reveals the molecular basis for catalytic priming.
    Nat Struct Mol Biol. 2013;20:696-701.

  44. Sigismund S, Confalonieri S, Ciliberto A, Polo S, Scita G and Di Fiore PP.
    Endocytosis and signaling: cell logistics shape the eukaryotic cell plan.
    Physiol Rev. 2012;92:273-366.

  45. Polo S.
    Signaling-mediated control of ubiquitin ligases in endocytosis.
    BMC Biol. 2012;10:25.

  46. Gatti M, Pinato S, Maspero E, Soffientini P, Polo S and Penengo L.
    A novel ubiquitin mark at the N-terminal tail of histone H2As targeted by RNF168 ubiquitin ligase.
    Cell Cycle. 2012;11:2538-44.

  47. Maspero E, Mari S, Valentini E, Musacchio A, Fish A, Pasqualato S and Polo S.
    Structure of the HECT: ubiquitin complex and its role in ubiquitin chain elongation.
    EMBO Rep. 2011;12:342-9.

  48. Inui M, Manfrin A, Mamidi A, Martello G, Morsut L, Soligo S, Enzo E, Moro S, Polo S, Dupont S, Cordenonsi M and Piccolo S.
    USP15 is a deubiquitylating enzyme for receptor-activated SMADs.
    Nat Cell Biol. 2011;13:1368-75.

  49. Argenzio E, Bange T, Oldrini B, Bianchi F, Peesari R, Mari S, Di Fiore PP, Mann M and Polo S.
    Proteomic snapshot of the EGF-induced ubiquitin network.
    Mol Syst Biol. 2011;7:462.

  50. Frosi Y, Anastasi S, Ballaro C, Varsano G, Castellani L, Maspero E, Polo S, Alema S and Segatto O.
    A two-tiered mechanism of EGFR inhibition by RALT/MIG6 via kinase suppression and receptor degradation.
    J Cell Biol. 2010;189:557-71.

  51. Lai FP, Szczodrak M, Oelkers JM, Ladwein M, Acconcia F, Benesch S, Auinger S, Faix J, Small JV, Polo S, Stradal TE and Rottner K.
    Cortactin promotes migration and platelet-derived growth factor-induced actin reorganization by signaling to Rho-GTPases.
    Mol Biol Cell. 2009;20:3209-23.

  52. Acconcia F, Sigismund S and Polo S.
    Ubiquitin in trafficking: the network at work.
    Exp Cell Res. 2009;315:1610-8.

  53. Sigismund S, Argenzio E, Tosoni D, Cavallaro E, Polo S and Di Fiore PP.
    Clathrin-mediated internalization is essential for sustained EGFR signaling but dispensable for degradation.
    Dev Cell. 2008;15:209-19.

  54. Polo S and Di Fiore PP.
    Finding the right partner: science or ART?
    Cell. 2008;135:590-2.

  55. Woelk T, Sigismund S, Penengo L and Polo S.
    The ubiquitination code: a signalling problem.
    Cell Div. 2007;2:11.

  56. Woelk T, Oldrini B, Maspero E, Confalonieri S, Cavallaro E, Di Fiore PP and Polo S.
    Molecular mechanisms of coupled monoubiquitination.
    Cell Biol. 2006;8:1246-54.

  57. Polo S and Di Fiore PP.
    Endocytosis conducts the cell signaling orchestra.
    Cell. 2006;124:897-900.

  58. Penengo L, Mapelli M, Murachelli AG, Confalonieri S, Magri L, Musacchio A, Di Fiore PP, Polo S and Schneider TR.
    Crystal structure of the ubiquitin binding domains of rabex-5 reveals two modes of interaction with ubiquitin.
    Cell. 2006;124:1183-95.

  59. Sigismund S, Woelk T, Puri C, Maspero E, Tacchetti C, Transidico P, Di Fiore PP and Polo S
    Clathrin-independent endocytosis of ubiquitinated cargos.
    Proc Natl Acad Sci U S A. 2005;102:2760-5.

  60. Mao Y, Senic-Matuglia F, Di Fiore PP, Polo S, Hodsdon ME and De Camilli P.
    Deubiquitinating function of ataxin-3: insights from the solution structure of the Josephin domain.
    Proc Natl Acad Sci U S A. 2005;102:12700-5.

  61. Innocenti M, Gerboth S, Rottner K, Lai FP, Hertzog M, Stradal TE, Frittoli E, Didry D, Polo S, Disanza A, Benesch S, Di Fiore PP, Carlier MF and Scita G.
    Abi1 regulates the activity of N-WASP and WAVE in distinct actin-based processes.
    Nat Cell Biol. 2005;7:969-76.

  62. Benesch S, Polo S, Lai FP, Anderson KI, Stradal TE, Wehland J and Rottner K.
    N-WASP deficiency impairs EGF internalization and actin assembly at clathrin-coated pits.
    J Cell Sci. 2005;118:3103-15.

  63. Sigismund S, Polo S and Di Fiore PP.
    Signaling through monoubiquitination.
    Curr Top Microbiol Immunol. 2004;286:149-85.

  64. Polo S, Pece S and Di Fiore PP.
    Endocytosis and cancer.
    Curr Opin Cell Biol. 2004;16:156-61.

  65. Polo S, Confalonieri S, Salcini AE and Di Fiore PP.
    EH and UIM: endocytosis and more.
    Sci STKE. 2003;2003:re17.

  66. Haglund K, Sigismund S, Polo S, Szymkiewicz I, Di Fiore PP and Dikic I.
    Multiple monoubiquitination of RTKs is sufficient for their endocytosis and degradation.
    Nat Cell Biol. 2003;5:461-6.

  67. Di Fiore PP, Polo S and Hofmann K.
    When ubiquitin meets ubiquitin receptors: a signalling connection.
    Nat Rev Mol Cell Biol. 2003;4:491-7.

  68. Chen H, Polo S, Di Fiore PP and De Camilli PV.
    Rapid Ca2+-dependent decrease of protein ubiquitination at synapses.
    Proc Natl Acad Sci U S A. 2003;100:14908-13.

  69. Bianchi E, Denti S, Catena R, Rossetti G, Polo S, Gasparian S, Putignano S, Rogge L and Pardi R.
    Characterization of human constitutive photomorphogenesis protein 1, a RING finger ubiquitin ligase that interacts with Jun transcription factors and modulates their transcriptional activity.
    J Biol Chem. 2003;278:19682-90.

  70. Poupon V, Polo S, Vecchi M, Martin G, Dautry-Varsat A, Cerf-Bensussan N, Di Fiore PP and Benmerah A.
    Differential nucleocytoplasmic trafficking between the related endocytic proteins Eps15 and Eps15R.
    J Biol Chem. 2002;277:8941-8.

  71. Polo S, Sigismund S, Faretta M, Guidi M, Capua MR, Bossi G, Chen H, De Camilli P and Di Fiore PP.
    A single motif responsible for ubiquitin recognition and monoubiquitination in endocytic proteins.
    Nature. 2002;416:451-5.


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