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Microscopia ottica avanzata

Microscopia Ottica Avanzata

Fabrizio Orsenigo

Tecnico

Fabrizio Orsenigo è uno specialista di microscopia con oltre 20 anni di esperienza in biologia molecolare, cellulare e murina, con un forte focus sulla microscopia ottica e l’analisi d’immagine. Ha iniziato la sua carriera all’Istituto Mario Negri, conseguendo il titolo di Tecnico di Ricerca nel 2001, per poi entrare in IFOM nel laboratorio di biologia vascolare guidato da Elisabetta Dejana. Ha contribuito a numerosi progetti di ricerca sull’angiogenesi e i microambienti tumorali.
Nel 2016 ha collaborato alla creazione del laboratorio della Dejana all’Università di Uppsala, coordinando l’installazione di sistemi avanzati di microscopia.
Dal 2022 fa parte dell’Unità di Microscopia Ottica Avanzata di IFOM, dove si occupa di imaging ad alta risoluzione e analisi con Fiji, QuPath, Arivis e Python. Partecipa inoltre alle attività formative, insegnando preparazione dei tessuti e analisi d’immagine di base agli studenti della SEMM.

Pubblicazioni

Publications Pre-IFOM:
  1. Corada, M., Zanetta, L., Orsenigo, F., […], Dejana, E. A monoclonal antibody to vascular endothelial-cadherin inhibits tumor angiogenesis without side effects on endothelial permeability. Blood 100, 905-911, doi:10.1182/blood.v100.3.905 (2002).
  2. Martinez-Estrada, O. M.,[…], Orsenigo, F., […], Bazzoni, G. Association of junctional adhesion molecule with calcium/calmodulin-dependent serine protein kinase (CASK/LIN-2) in human epithelial caco-2 cells. J Biol Chem 276, 9291-9296, doi:10.1074/jbc.M006991200 (2001).
  3. Bazzoni, G., Martinez-Estrada, O. M., Orsenigo, F.,[…], Dejana, E. Interaction of junctional adhesion molecule with the tight junction components ZO-1, cingulin, and occludin. J Biol Chem 275, 20520-20526, doi:10.1074/jbc.M905251199 (2000).
Publications IFOM:
  1. Onyeogaziri, F. C., […], Orsenigo, F., […], Magnusson, P. U. Pharmacological blocking of neutrophil extracellular traps attenuates immunothrombosis and neuroinflammation in cerebral cavernous malformation. Nat Cardiovasc Res 3, 1549-1567, doi:10.1038/s44161-024-00577-y (2024).
  2. Nespolo, A., […], Orsenigo, F, […], Sonego, M. USP1 deubiquitinates PARP1 to regulate its trapping and PARylation activity. Sci Adv 10, eadp6567, doi:10.1126/sciadv.adp6567 (2024).
  3. Bastianello, G., […], Orsenigo, F., […], Foiani, M. Mechanical stress during confined migration causes aberrant mitoses and c-MYC amplification. Proc Natl Acad Sci U S A 121, e2404551121, doi:10.1073/pnas.2404551121 (2024).
  4. Panebianco, C., […], Orsenigo, F., […], Pazienza, V. Counteracting gemcitabine+nab-paclitaxel induced dysbiosis in KRAS wild type and KRAS(G12D) mutated pancreatic cancer in vivo model. Cell Death Discov 9, 116, doi:10.1038/s41420-023-01397-y (2023).
  5. Hershey, B. J., Barozzi, S., Orsenigo, F., […], Havas, K. M. Clonal cooperation through soluble metabolite exchange facilitates metastatic outgrowth by modulating Allee effect. Sci Adv 9, eadh4184, doi:10.1126/sciadv.adh4184 (2023).
  6. Beter, M., […], Orsenigo, F., […], Laakkonen, J. P. SproutAngio: an open-source bioimage informatics tool for quantitative analysis of sprouting angiogenesis and lumen space. Sci Rep 13, 7279, doi:10.1038/s41598-023-33090-6 (2023).
  7. Yau, A. C. Y., […], Orsenigo, F., […], Dejana, E. & Magnusson, P. U. Inflammation and neutrophil extracellular traps in cerebral cavernous malformation. Cell Mol Life Sci 79, 206, doi:10.1007/s00018-022-04224-2 (2022).
  8. Panebianco, C., […], Orsenigo, F., […], Pazienza, V. Butyrate, a postbiotic of intestinal bacteria, affects pancreatic cancer and gemcitabine response in in vitro and in vivo models. Biomed Pharmacother 151, 113163, doi:10.1016/j.biopha.2022.113163 (2022).
  9. Globisch, M. A., […], Orsenigo, F., […], Magnusson, P. U. Immunothrombosis and vascular heterogeneity in cerebral cavernous malformation. Blood 140, 2154-2169, doi:10.1182/blood.2021015350 (2022).
  10. Oldenburg, J., […], Orsenigo, F., […], Magnusson, P. U. Propranolol Reduces the Development of Lesions and Rescues Barrier Function in Cerebral Cavernous Malformations: A Preclinical Study. Stroke 52, 1418-1427, doi:10.1161/STROKEAHA.120.029676 (2021).
  11. Combes, T. W., Orsenigo, F., […], Martinez, F. O. CSF1R defines the mononuclear phagocyte system lineage in human blood in health and COVID-19. Immunother Adv 1, ltab003, doi:10.1093/immadv/ltab003 (2021).
  12. Orsenigo, F., Conze, L. L., […], Dejana, E. Mapping endothelial-cell diversity in cerebral cavernous malformations at single-cell resolution. Elife 9, doi:10.7554/eLife.61413 (2020).
  13. Malinverno, M., […], Orsenigo, F., […], Dejana, E. Endothelial cell clonal expansion in the development of cerebral cavernous malformations. Nat Commun 10, 2761, doi:10.1038/s41467-019-10707-x (2019).
  14. Corada, M., Orsenigo, F., […], Dejana, E. Fine-Tuning of Sox17 and Canonical Wnt Coordinates the Permeability Properties of the Blood-Brain Barrier. Circ Res 124, 511-525, doi:10.1161/CIRCRESAHA.118.313316 (2019).
  15. Conway, D. E., […], Orsenigo, F., […], Schwartz, M. A. VE-Cadherin Phosphorylation Regulates Endothelial Fluid Shear Stress Responses through the Polarity Protein LGN. Curr Biol 27, 2219-2225 e2215, doi:10.1016/j.cub.2017.06.020 (2017).
  16. Gordon, E. J., Fukuhara, D., Westrom, S., Padhan, N., Sjostrom, E. O., van Meeteren, L., He, L., Orsenigo, F., Dejana, E., Bentley, K., Spurkland, A. & Claesson-Welsh, L. The endothelial adaptor molecule TSAd is required for VEGF-induced angiogenic sprouting through junctional c-Src activation. Sci Signal 9, ra72, doi:10.1126/scisignal.aad9256 (2016).
  17. Giampietro, C., […], Orsenigo, F., […], Ghigna, C. The alternative splicing factor Nova2 regulates vascular development and lumen formation. Nat Commun 6, 8479, doi:10.1038/ncomms9479 (2015).
  18. Zheng, W., […], Orsenigo, F., […], Alitalo, K. Angiopoietin 2 regulates the transformation and integrity of lymphatic endothelial cell junctions. Genes Dev 28, 1592-1603, doi:10.1101/gad.237677.114 (2014).
  19. Moreno, V., […], Orsenigo, F., […], Arroyo, A. G. An EMMPRIN-gamma-catenin-Nm23 complex drives ATP production and actomyosin contractility at endothelial junctions. J Cell Sci 127, 3768-3781, doi:10.1242/jcs.149518 (2014).
  20. Goddard, L. M., […], Orsenigo, F., […], Iruela-Arispe, M. L. Progesterone receptor in the vascular endothelium triggers physiological uterine permeability preimplantation. Cell 156, 549-562, doi:10.1016/j.cell.2013.12.025 (2014).
  21. Giannotta, M., […], Orsenigo, F., […], Dejana, E. Targeting endothelial junctional adhesion molecule-A/ EPAC/ Rap-1 axis as a novel strategy to increase stem cell engraftment in dystrophic muscles. EMBO Mol Med 6, 239-258, doi:10.1002/emmm.201302520 (2014).
  22. Maddaluno, L., […], Orsenigo, F., […], Dejana, E. EndMT contributes to the onset and progression of cerebral cavernous malformations. Nature 498, 492-496, doi:10.1038/nature12207 (2013).
  23. Franco, D., […], Orsenigo, F., […], Kurtcuoglu, V. Accelerated endothelial wound healing on microstructured substrates under flow. Biomaterials 34, 1488-1497, doi:10.1016/j.biomaterials.2012.10.007 (2013).
  24. Dejana, E. & Orsenigo, F. Endothelial adherens junctions at a glance. J Cell Sci 126, 2545-2549, doi:10.1242/jcs.124529 (2013).
  25. Corada, M., Orsenigo, F., […], Dejana, E. Sox17 is indispensable for acquisition and maintenance of arterial identity. Nat Commun 4, 2609, doi:10.1038/ncomms3609 (2013).
  26. Anisimov, A., […], Orsenigo, F., […], Alitalo, K. Vascular endothelial growth factor-angiopoietin chimera with improved properties for therapeutic angiogenesis. Circulation 127, 424-434, doi:10.1161/CIRCULATIONAHA.112.127472 (2013).
  27. Orsenigo, F., Giampietro, C., […], Dejana, E. Phosphorylation of VE-cadherin is modulated by haemodynamic forces and contributes to the regulation of vascular permeability in vivo. Nat Commun 3, 1208, doi:10.1038/ncomms2199 (2012).
  28. Giampietro, C., […], Orsenigo, F., […], Dejana, E. Overlapping and divergent signaling pathways of N-cadherin and VE-cadherin in endothelial cells. Blood 119, 2159-2170, doi:10.1182/blood-2011-09-381012 (2012).
  29. Paolinelli, R., Corada, M., Orsenigo, F. & Dejana, E. The molecular basis of the blood brain barrier differentiation and maintenance. Is it still a mystery? Pharmacol Res 63, 165-171, doi:10.1016/j.phrs.2010.11.012 (2011).
  30. Murakami, M., […], Orsenigo, F., […], Dejana, E. Abrogation of junctional adhesion molecule-A expression induces cell apoptosis and reduces breast cancer progression. PLoS One 6, e21242, doi:10.1371/journal.pone.0021242 (2011).
  31. Lampugnani, M. G., Orsenigo, F., […], Dejana, E. CCM1 regulates vascular-lumen organization by inducing endothelial polarity. J Cell Sci 123, 1073-1080, doi:10.1242/jcs.059329 (2010).
  32. Hermans, K., […], Orsenigo, F., […], Dejana, E., Alitalo, K. & Carmeliet, P. Role of synectin in lymphatic development in zebrafish and frogs. Blood 116, 3356-3366, doi:10.1182/blood-2009-11-254557 (2010).
  33. Corada, M., Nyqvist, D., Orsenigo, F., […], Dejana, E. The Wnt/beta-catenin pathway modulates vascular remodeling and specification by upregulating Dll4/Notch signaling. Dev Cell 18, 938-949, doi:10.1016/j.devcel.2010.05.006 (2010).
  34. Montero-Balaguer, M., Swirsding, K., Orsenigo, F., […], Dejana, E. Stable vascular connections and remodeling require full expression of VE-cadherin in zebrafish embryos. PLoS One 4, e5772, doi:10.1371/journal.pone.0005772 (2009).
  35. Hosking, B., […], Orsenigo, F., […], Koopman, P. Sox7 and Sox17 are strain-specific modifiers of the lymphangiogenic defects caused by Sox18 dysfunction in mice. Development 136, 2385-2391, doi:10.1242/dev.034827 (2009).
  36. Dejana, E., Orsenigo, F., […], McDonald, D. M. Organization and signaling of endothelial cell-to-cell junctions in various regions of the blood and lymphatic vascular trees. Cell Tissue Res 335, 17-25, doi:10.1007/s00441-008-0694-5 (2009).
  37. Cera, M. R., […], Orsenigo, F., […], Dejana, E. JAM-A promotes neutrophil chemotaxis by controlling integrin internalization and recycling. J Cell Sci 122, 268-277, doi:10.1242/jcs.037127 (2009).
  38. Taddei, A., […], Orsenigo, F., […], Dejana, E. Endothelial adherens junctions control tight junctions by VE-cadherin-mediated upregulation of claudin-5. Nat Cell Biol 10, 923-934, doi:10.1038/ncb1752 (2008).
  39. Francois, M., […], Orsenigo, F., […], Koopman, P. Sox18 induces development of the lymphatic vasculature in mice. Nature 456, 643-647, doi:10.1038/nature07391 (2008).
  40. Dejana, E., Orsenigo, F. & Lampugnani, M. G. The role of adherens junctions and VE-cadherin in the control of vascular permeability. J Cell Sci 121, 2115-2122, doi:10.1242/jcs.017897 (2008).
  41. Lampugnani, M. G., Orsenigo, F., […], Dejana, E. Vascular endothelial cadherin controls VEGFR-2 internalization and signaling from intracellular compartments. J Cell Biol 174, 593-604, doi:10.1083/jcb.200602080 (2006).
  42. Spagnuolo, R., Corada, M., Orsenigo, F., […], Dejana, E. Gas1 is induced by VE-cadherin and vascular endothelial growth factor and inhibits endothelial cell apoptosis. Blood 103, 3005-3012, doi:10.1182/blood-2003-07-2459 (2004).
  43. Grazia Lampugnani, M., […], Orsenigo, F., […], Dejana, E. Contact inhibition of VEGF-induced proliferation requires vascular endothelial cadherin, beta-catenin, and the phosphatase DEP-1/CD148. J Cell Biol 161, 793-804, doi:10.1083/jcb.200209019 (2003).
  44. Lampugnani, M. G., […], Orsenigo, F., […], Dejana, E. VE-cadherin regulates endothelial actin activating Rac and increasing membrane association of Tiam. Mol Biol Cell 13, 1175-1189, doi:10.1091/mbc.01-07-0368 (2002).
Fabrizio Orsenigo

Facility members

Dario Parazzoli, Responsabile della Core Facility

Sara Barozzi

Zeno Lavagnino

Serena Magni

Mattia Pavani

Reference PI:

Giorgio Scita