Microscopia ottica avanzata
Zeno Lavagnino
Tecnico
Zeno Lavagnino ha conseguito la laurea in Fisica presso l’Università di Genova nel 2010.
Ha iniziato a occuparsi di microscopia ottica durante il dottorato nel laboratorio di Alberto Diaspro all’Istituto Italiano di Tecnologia, dove ha sviluppato microscopi che combinano imaging a singola molecola, eccitazione a due fotoni e illuminazione light-sheet.
L'interesse per la biofisica lo ha portato a un postdoc nel laboratorio di David Piston alla Washington University di St. Louis, dove ha costruito e ottimizzato un microscopio light-sheet spettrale per lo studio delle isole pancreatiche.
Nel 2018 ha ottenuto una Marie Curie Fellowship per rientrare in Italia, nel laboratorio di Davide Mazza all'Ospedale San Raffaele, integrando tecniche di light-sheet a singolo obiettivo, tracciamento di singole molecole e illuminazione strutturata.
Dal 2021 fa parte della IFOM Advanced Light Microscopy Facility, dove applica tecniche avanzate di microscopia alla ricerca sul cancro.
Publications Pre-IFOM
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Mazzocca M, ….. Lavagnino Z. , Tacchetti C., Mazza D.
"Chromatin organization drives the search mechanism of nuclear factors"
Nat. Comm. 14(1) (2023) - Abreu D, Asada R. Revilla JMP, Lavagnino Z, Kries K, Piston, DW, Urano F.
"Wolfram syndrome 1 gene regulates pathways maintaining beta-cell health and survival"
Laboratory investigation, 100(6), p.849-862 (2020) -
Verma M, Choi J, Cottrell KA, Lavagnino Z, Thomas EN, Pavlovic-Djuranovic S, Szczesny P, Piston DW, Zaher HS, Puglisi JD, Djuranovic S.
"A short translational ramp determines the efficiency of protein synthesis."
Nat Commun. 10(1):5774 (2019) -
Li S, Lavagnino Z, Lemacon D, Kong L , Ustione A, Zhang Y, Wang Y, Zheng B, Piwnica-Worms H, Vindigni A, Piston DW, You Z.
"The Ca2+-CaMKK2-AMPK Axis Protects Stressed Replication Forks"
Mol Cell. 20;74(6):1123-113(2019) -
Ferri G, Digiacomo L, Lavagnino Z, Occhipinti M, Bugliani M, Caracciolo G, Marchetti P, Piston DW, Cardarelli F.
"Fingerprinting insulin secretory granules in living β-cells by fast spatiotemporal fluctuation spectroscopy"
Scientific Reports 9(1):2890 (2019) -
Hughes JW, Ustione A, Lavagnino Z, Piston DW
"Regulation of islet glucagon secretion: Beyond calcium"
Diabetes Obes Metab. Suppl 2: 127-136 (2018) -
Clark AL, Kanekura K, Lavagnino Z, Spears LD, Abreu D, Mahadevan J, Yagi T, Semenkovich CF, Piston DW, Urano F
"Targeting Cellular Calcium Homeostasis to Prevent Cytokine-Mediated Beta Cell Death"
Sci Reports; 7(1), (2017) -
Lee J, Harris AN, Holley CL, Mahadevan J, Pyles KH, Lavagnino Z, Scherrer DE, Fujiwara H, Sidhu R, Zhang J, Huang SCC, Piston DW, Remedi MS, Urano F, Ory DS, Schaffer JE.
" Rpl13a small nucleolar RNAs regulate systemic glucose metabolism",
Journal of Clinical Investigation, 126(12), (2016) -
Lavagnino Z, Dwight J, Ustione A, Thuc-Uyen N, Tkaczyk TS, Piston DW
"Snapshot hyperspectral light-sheet imaging of signal transduction dynamics in in live pancreatic islets"
Biophysical Journal, 111(2), (2016). -
Cranfill P, Sell BR, Baird MA, Allen JR, Lavagnino Z, de Gruiter HM, Kremers GJ, Davidson MW, Ustione A, Piston DW
"Quantitative assessment of fluorescent proteins"
Nature Methods, 13, (2016) -
Lavagnino Z, Sancataldo G, d’Amora M, Follert P, De Pietri Tonelli D, Diaspro A, Cella Zanacchi F.
"4D (x-y-z-t) imaging of thick biological samples by means of Two-Photon inverted Selective Plane Illumination Microscopy (2PE-iSPIM)"
Sci Reports 6, 23923 (2016) -
Cella Zanacchi F, Lavagnino Z, Faretta M, Furia L, Diaspro A.
" Light-Sheet Confined Super-Resolution Using Two-Photon Photoactivation"
Plos One, 8 (7) (2013) -
Lavagnino Z, Cella Zanacchi F., Ronzitti E., Diaspro A.
"Two-photon excitation selective plane illumination microscopy (2PE-SPIM) of highly scattering samples: characterization and application"
Optics Express 21(5), 5998-6008 (2013) -
Cella Zanacchi F, Lavagnino Z, Perrone Donnorso M, Del Bue A, Furia L, Faretta M, Diaspro A
"Live-cell 3D super-resolution imaging in thick biological samples"
Nature Methods 8, 1047-1049 (2011)
Publications IFOM
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Ghisleni A., Bonilla-Quintana M., … Lavagnino Z., … Gauthier N.C.
“Mechanically induced topological transition of spectrin regulates its distribution in the mammalian cell cortex”
Nature Communications, 15(1), 5711 (2024) - Popęda M., Kowalski K.,….. Lavagnino Z., … Maiuri P., Nastaly P.
“Emerin mislocalization during chromatin bridge resolution can drive prostate cancer cell invasiveness in a collagen-rich microenvironment”
Experimental and Molecular Medicine, 56(9), pp. 2016–2032 (2024) - Rosso I., Jones-Weinert C., ….. Lavagnino Z.,… d’Adda di Fagagna F.
“Alterntive lengthening of telomeres (ALT) cells viability is dependent on C-rich telomeric RNAs”
Nat. Comm 14(1) (2023) - Gioia U., Tavella S.,… Lavagnino Z.,… d’Adda di Fagagna F.
“SARS-CoV-2 infection induces DNA damage, through CHK1 degradation and impaired 53BP1 recruitment, and cellular senescence”
Nature Cell Biology, 25(4), pp. 550–564 (2023) - Frittoli E.,Palamidessi A., … Lavagnino Z,….Scita G.
“Tissue fluidification promotes a cGAS–STING cytosolic DNA response in invasive breast cancer”
Nat Materials 22(5) (2023)
